<\/a>Technicians at the Alaska State Crime Lab recently identified the human remains found in a shoe that washed up near the Seawalk in 2020 by identifying the deceased’s DNA. (Courtesy photo \/ Department of Public Safety)<\/p><\/div>\n\t\t\t\t
“We would not receive the entire foot we would receive a sample of the tissue present to take through our process. What we really need is deep muscle tissue (like thigh muscle) for the best chance of getting a comparable DNA profile,” Foster said. “In this case with the foot that was recovered the DNA present would likely be compromised due to the fact that it was submerged in water.”<\/p>\n\t\t\t\t
Exposure to the elements and to the animals in it degrades the remains and the chances of getting a good DNA profile, Foster said.<\/p>\n\t\t\t\t
“Remains that are recovered after being out in the elements are very degraded and the best chance of getting a profile will be from the bones\/teeth and not soft tissue,” Foster said. “Once we have a sample we take it through out normal DNA procedures.”<\/p>\n\t\t\t\t
The normal DNA procedures have four steps, Foster said: extraction, quantification, amplification and analysis\/interpretation.<\/p>\n\t\t\t\t
“Extraction, isolating the DNA from the cells and ‘cleaning’” it up — getting rid of cellular debris and other things. Quantification, which tells us how much DNA we have in the sample, if it is degraded and if we have enough to go forward in the process,” Foster said. “Amplification, making millions of coping of the DNA present. Analysis\/Interpretation, using capillary electrophoresis to separate the DNA pieces by size and generate a profile.”<\/p>\n\t\t\t\t
If a DNA profile is able to be generated, Foster said, it’s uploaded to the Combined DNA Index System, a program run by the FBI maintaining a wide variety of DNA databases that can be searched against with the profile that’s been generated. It might also be compared a sample from a known relative of a possible missing person.<\/p>\n\t\t\t\t
“If there is an idea of who the remains may belong to and known samples are able to be collected from biological parents or children, we’re able to make this type of comparison,” Foster said. “(The Alaska state crime lab is) not able to do a sibship comparison (comparing the profile to biological siblings), but this can be done and can be sent out to a lab that is able to make that type of comparison.”<\/p>\n\t\t\t\t
The processing takes a series of weeks, following a review of the case, Foster said. Human remains cases are fast-tracked, Foster said, giving them priority as they come in. Not every set of remains generates a result, Foster said; in those cases, she consults with the law enforcement agencies and the medical examiner to find another option for identification.<\/p>\n\t\t\t\t
• Contact reporter Michael S. Lockett at (757) 621-1197 or mlockett@juneauempire.com.<\/em><\/p>\n\t\t\t","protected":false},"excerpt":{"rendered":"Deep muscle tissue is best for identifying workable DNA. <\/p>\n","protected":false},"author":534,"featured_media":79348,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_stopmodifiedupdate":false,"_modified_date":"","wds_primary_category":9,"footnotes":""},"categories":[9,4],"tags":[59,75,230],"yst_prominent_words":[],"class_list":["post-79347","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-home","category-news","tag-crime","tag-local-news","tag-state-news"],"_links":{"self":[{"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/posts\/79347","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/users\/534"}],"replies":[{"embeddable":true,"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/comments?post=79347"}],"version-history":[{"count":0,"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/posts\/79347\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/media\/79348"}],"wp:attachment":[{"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/media?parent=79347"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/categories?post=79347"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/tags?post=79347"},{"taxonomy":"yst_prominent_words","embeddable":true,"href":"https:\/\/www.juneauempire.com\/wp-json\/wp\/v2\/yst_prominent_words?post=79347"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}